Methidiumpropyl-edta
WebMethidiumpropyl-EDTA (MPE) cleaves DNA efficiently and with low sequence specificity in the presence of Fe(II), 02 and reducing agents (15,16). MPE.Fe(II) footprinting has been … WebSigma, and methidiumpropyl-EDTA (12, 20, 21) was gra-ciously provided by Peter B. Dervan (California Institute of Technology, Pasadena). [y-32P]rATP and [a-32P]dCTP …
Methidiumpropyl-edta
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WebA simple bifunctional molecule, methidiumpropyl-EDTA (MPE), which contains the DNA intercalator methidium covalently bound by a short hydrocarbon tether to the metal … Web21 mei 2012 · Abstract. Using two direct methods we have studied the binding locations and site sizes of distamycin and penta-N-methylpyrrolecarboxamide on three DNA restriction …
WebMethidiumpropyl-EDTA.FE(II) footprinting confirms minimal contacts between polymerase and DNA. We infer that polymerase is directed to the promoter by a DNA sequence … Web1 okt. 1991 · The Mu A protein is responsible for the structural and functional integrity of the Type 1 transpososome. We have investigated the quaternary structure of the Mu A protein within this complex by chemical cross-linking experiments and found that the basic structural unit is an A tetramer.
Web1 jul. 1985 · Van Dyke MW, Dervan PB. Methidiumpropyl-EDTA.Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA. Nucleic Acids Res. … WebHere we sought to evaluate the contribution of the PBD unit to the biological activity of PBD-conjugates and, to this end, an adenosine nucleoside was attached to the PBD A-ring C8 …
Web25 aug. 1983 · Methidiumpropyl-EDTA-Fe(II) and DNase I footprinting report different small molecule binding site sizes on DNA Nucleic Acids Research Oxford Academic …
WebPublisher Summary This chapter discusses the analysis of nucleic acids in gels using glyoxal and acridine orange. To determine the molecular weights of nucleic acids using … omni manufacturing services queretaroWeb7 jul. 2015 · Here we describe methidiumpropyl-EDTA sequencing (MPE-seq), a method for the genome-wide characterization of chromatin that involves the digestion of nuclei … omni man vs homelander reactionWebreagent and so provides low resolution, and (methidiumpropyl-EDTA)-Fe(I1) intercalates into DNA and is therefore biased toward cleavage of double-stranded DNA. In this study, … is arthur bad for kidsWeb25 jun. 1991 · Data suggest that the loss in transcriptional activity by the foot mutant results from a severe decrease in processivity as well as catalytic efficiency of the enzyme. The … isarthroseWeb19 feb. 2024 · EDTA methidiumpropylamide (Q27126421) chemical compound methidiumpropyl-EDTA edit Statements instance of chemical compound 0 references … omni man vs s class heroesWebPublisher Summary This chapter discusses the analysis of nucleic acids in gels using glyoxal and acridine orange. To determine the molecular weights of nucleic acids using gel electrophoresis techniques, the molecules must have equivalent conformations. This is achieved by removing native secondary and tertiary structure with a variety of chemical … omni man the boysWebMethidiumpropyl-EDTA (MPE) cleaves DNA efficiently in the presence of Fe(II), 02, and reducing agents such as dithiothreitol (14). Importantly, MPE'Fe(II) cleaves DNA with low … is arthur an aardvark