TīmeklisPirms 2 dienām · Illumina Stranded RNA library preparation with RiboZero Plus rRNA depletion and pair-ended Illumina sequencing (2x51bp) were performed at the Microbial Genome Sequencing Center (Pittsburgh, PA). TīmeklisRibo-Zero Plus rRNA Depletion Kit. 説明. メタトランスクリプトミクス研究のための宿主および細菌rRNAからの大量の転写産物の枯渇。. 複数の種または転写産物からの …
RIBO Zero Reference Guide - Illumina, Inc.
TīmeklisRNA sequencing libraries were prepared using Ribo-Zero rRNA Removal Kit (H/M/R) (Illumina Cat#: MRZH11124) and NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (Cat#: E7420). The total RNA input amount for the RiboZero kit was 1 m total. The rRNA input for library construction was 50 ng total. Libraries were amplified for … TīmeklisIn order to prepare RNA samples for whole-transcriptome analysis from a source that is not animal, plant, or bacteria, use the Epicentre's MatchMaker Tool™ to test Ribo … shortcut split video
Illumina Ribo-Zero Plus rRNA Depletion Kit
TīmeklisRibo-Zero kits can be used with TruSeq Stranded mRNA kits to create libraries. Starting Material: Begin RiboZero with 1-5ug total RNA, then take 10-100ng RiboZero product into TruSeq protocol (see second note in "Purify and Fragment mRNA" section of the User Guide). LT HT TruSeq Stranded mRNA Catalog ID (s): TīmeklisIllumina RiboZero Gold (RZ), Lexogen RiboCop (LX), Qiagen GeneRead rRNA Depletion (Q), all of which use bead capture for ribosomal depletion, the New England Biolabs NEBNext rRNA Depletion (NE), Kapa RiboErase (K), and Takara/Clontech Ribogone (CR) kits that are based on RNAseH under aCC-BY-NC-ND 4.0 … TīmeklisRNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, … shortcut split screen windows 10